Charles Grose, MD
Peer Review Status: Internally Peer Reviewed

Laboratory Hours and Location

Location: The University of Iowa Hospitals' Viral Diagnostic Laboratory is located in 265 Medical Research Center

Telephone Number: 356-4463

Hours: The University of Iowa Hospitals Viral Diagnostic Laboratory is open from 8:00 am to 4:30 pm Monday through Friday and from 8:00 to 11:00 am Saturday and Sundays. Specimens should be in the laboratory no later than 4:00 pm Monday through Friday for processing.

Receipt of Specimens After Hours: Every effort should be made to get specimens to the laboratory on the same day they are collected (by 4:00 pm). Overnight storage of specimens, especially urines and tissues, should be avoided; it is preferable to obtain the specimen early the next morning. When this is not feasible, specimens should be taken after 4:00 pm M-F and 0800 to 0800 S-S to 6248 RCP, Specimen Control. All specimens must be on ice.

Transport Media

Collection of the specimen into appropriate transport media is essential to ensure viability of the organisms to be cultured.

Transport Systems

Swabs: Virocult; Chlamydia Transwab
Available from Hospital Stores during regular working hours. Specimen Control during off-hours.

Tissue: Broth medium available in the Virology Lab, Specimen Control, 6248 RCP, Frozen Section Room.

Specimen Collection Guidelines For Clinical Virology Lab

Send all specimens to 265 MRC or Specimen Control 6248 RCP ON ICE.

Procedures Available:

Isolation and Identification of Herpes simplex, cytomegalovirus, varicella-zoster, respiratory syncytial virus, Chlamydia trachomatis; rotavirus antigen detection; varicella-zoster immune status; and rubella immune status.

Do not clean area to be cultured with alcohol.

Viral

  • Herpesviruses (CMV, HSV, VZV)
  • Vesicle Fluid: Virocult swab of fluid obtained from disrupted vesicle or lesion.
  • Spinal Fluid: Aseptic collection into sterile containers.
  • Biopsy and autopsy tissue: Immerse tissue in broth transport media.
  • Ulcerative lesions: Swabs (anal, genital, ocular). Use Virocult swab and scrape surface of lesion.
  • Urine: Freshly passed AM urine is preferable. Collect clean voided midstream sample or suprapubic aspirate in sterile container. At least two or three specimens should be obtained to maximize recovery of CMV.
  • Blood:
    • Rubella and Varicella serology: Submit at least 2 ml of clotted whole blood (red top tube) not necessary to hand carry or put on ice.
    • Buffy coat culture: Submit at least 1 ml of heparinized blood (green top tube).
     
  • Respiratory Syncytial Virus: NP Washings, Tracheal aspirates, sputum; Throat swab as last resort.
  • Human Immunodeficiency Virus (HIV)
    • Blood
      HIV serology. Submit at least 2 ml of clotted whole blood (red top tube).
    • Culture.
      Call State Hygienic Laboratory in Iowa City to obtain current instructions about methods for culturing HIV from blood or other specimens. Remember to obtain consent forms prior to submitting specimens.
     

Chlamydia (trachomatis)
For best results specimens should be aseptically collected with some vigor (swabbing or scraping) to insure an adequate number of epithelial cells in collection media.

  • Swab: sterile Chlamydia Transwab * Conjunctiva: firmly stroke the everted lower eyelid with swab; place swab into transport media and transport to the lab on ice.
  • Nasopharyngeal Wash: (Transwab acceptable)
    • Draw approximately 1-3 ml phosphate buffered saline (PBS) into an infant bulb syringe (or normal saline without preservatives).
     
    • Inject fluid into nostrils carefully.
    • Aspirate fluid from nostril into bulb syringe. Place contents into sterile tube.
     
  • Urine: Not suitable for Chlamydia culture.

Rotavirus Antigen Detection
Liquid fecal specimen in sterile container. Swabs not acceptable.

Transport of Specimens

Specimen Transport:

Carry all specimens ON ICE to:
Virology Lab 265 MRC M-F 8 a.m. - 4:30 p.m.
Specimen Control 6248 RCP all other times.

Storage of Specimens After Hours:

Viral lability away from living cells necessitates that all viral specimens be transported on ice as rapidly as possible to the laboratory. When a specimen must be obtained after the laboratory is closed, specimens should be refrigerated at 4°C, if storage is less than or equal to 48 hrs. Otherwise specimens should be stored frozen at -70° C. Exceptions are urine specimens and tissue specimens which should not be frozen and ideally should be obtained the day they are to be cultured.

Transport of Specimens Table
Clinical Disease Commonly Associated with Virus Clinical Specimens Transport System
HSV - I, IITable cellTable cell Mouth + Lips:
  • gingivostomatitis
  • recurrent herpes
  • labialis
 
vesicle fluid or swab; throat swab Virocult
Eye:
  • acute follicular
  • conjunctivitis
  • corneal ulceration
  • stromal keratitis
 
vesicle fluid or swab; Virocult
CNS:
encephalitis
Brain bx*; CSF; HSV PCR
throat swab
Tissue Transport Media; Virocult
aseptic meningitis Buffy coat Green top tube
Skin:
vesicle
vesicle fluid* or swab  
Generalized Infections:
newborn
urine*; Buffy coat*; biopsy; vesicle swab*; throat swab*; bronchial washings; rectal swab*; conjunctivae Heparinized (green top) tube
Virocult
VZV Chickenpox vesicle fluid Virocult
Zoster lesion swab or fluid (CSF) Virocult
Generalized Infections:
newborn (pregnant mother with chickenpox)
serum Red top tube
throat swab, vesicle swab* or fluid, CSF*, urine, bronchial washings Virocult
CMV Generalized Infections:
newborn
urine*, buffy coat; Green top tube,
saliva, CSF Virocult
CNS:
infections
CSF, urine*  
Other:
hepatitis, pneumonia, mononucleosis
urine*, buffy coat; Green top tube,
biopsy Tissue Transport Media
Chlamydia Trachomatis Eye:
neonate and adult conjunctivitis
swab* Chlamydia transport media
Respiratory:
neonate pneumonia
nasal wash*; Chlamydia transport media
? adult pneumonia nasopharyngeal swab  

* Recommended specimen for optimal growth in culture